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DOI Prefix: 10.47001/IRJIET

Evaluation of Natural Dye for DNA Visualization in Agarose Gel

Sharada S BDepartment of Biotechnology, Basaveshwar Engineering College, Bagalkot, IndiaArun KulkarniDepartment of Biotechnology, Basaveshwar Engineering College, Bagalkot, IndiaHrishikesh H PattihalDepartment of Biotechnology, Basaveshwar Engineering College, Bagalkot, India

Vol 10 No 4 (2026): Volume 10, Issue 4, April 2026 | Pages: 363-369

International Research Journal of Innovations in Engineering and Technology

OPEN ACCESS | Research Article | Published Date: 30-04-2026

doi Logo doi.org/10.47001/IRJIET/2026.104051

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Abstract

Public Curcumin, a natural polyphenol extracted from turmeric, has been explored as a novel DNA stain for molecular biology applications. This study aims to extract the curcumin from turmeric and the extracted curcumin is confirmed by analytical techniques. Further the curcumin is studied for its application as natural dye for staining the DNA in Agarose gel. A solvent extraction method using ethanol was employed, followed by purification using column chromatography. The purified curcumin was characterized using HPLC and spectroscopic techniques. The extracted curcumin was then evaluated as a DNA stain for agarose gel electrophoresis, demonstrating superior sensitivity and specificity compared to conventional DNA stains. This study provides a robust and efficient method for extracting and purifying curcumin for DNA visualization, paving the way for its potential applications in molecular biology and genomics.

Keywords

Agarose gel, Ethidium bromide, Curcumin, natural dye, molecular biology


Citation of this Article

Sharada S B, Arun Kulkarni, & Hrishikesh H Pattihal. (2026). Evaluation of Natural Dye for DNA Visualization in Agarose Gel. International Research Journal of Innovations in Engineering and Technology - IRJIET, 10(4), 363-369. Article DOI https://doi.org/10.47001/IRJIET/2026.104051

Licence Copyright (c) 2026 International Research Journal of Innovations in Engineering and Technology creative common licence This work is licensed under Creative common Attribution Non Commercial 4.0 Internation Licence
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