International Research Journal of Innovations in Engineering and Technology - IRJIET International Open Access, Monthly, Peer Reviewed, Reputed Journal ISSN (online): 2581-3048

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DOI Prefix: 10.47001/IRJIET

Isolation of Flavonoid (Z)-2-(3-Acetyl-4-Hydroxy Benzylidine)-6-Hydroxy-4-Methoxybenzofuran-3(2h)-One of Rheum Rhabarbarum and Evaluation of Antimicrobial Activity against a Panel of Human Pathogens

Hala I ElzubirDepartment of Physical Sciences, Chemistry Division, College of Science, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaAbdel Karim MSudan University of Science and Technology, Faculty of Science, SudanAsra Banu SyedaDepartment of Physical Sciences, Chemistry Division, College of Science, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaLeena S IbrahimDepartment of Physical Sciences, Chemistry Division, College of Science, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaFatima A KhleefDepartment of Physical Sciences, Chemistry Division, College of Science, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaHiam M. OsmanDepartment of Physical Sciences, Chemistry Division, College of Science, Jazan University, P.O. Box 114, Jazan 45142, Saudi Arabia

Vol 10 No 4 (2026): Volume 10, Issue 4, April 2026 | Pages: 347-354

International Research Journal of Innovations in Engineering and Technology

OPEN ACCESS | Research Article | Published Date: 30-04-2026

doi Logo doi.org/10.47001/IRJIET/2026.104049

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Abstract

Rheum rhabarbarum, often referred to as rhubarb, has been utilized as a healing herb in various nations.  Particularly, its stems are recognized as a traditional remedy in various cultures. Rhubarbs contain numerous bioactive substances, belonging to diverse groups of phytochemicals, e.g. stilbenes, anthraquinones and flavonoids. In this study Phytochemical screening of Rheum rhabarbarum stems revealed the presence of flavonoids, alkaloids, sterols, saponins and glycosides. Stems Rheum rhabarbarum were macerated with 95% ethanol to prepare for isolation. The method used for isolation was a combination column chromatography (CC) followed by thin layer chromatography (TLC), which gave flavonoid (z)-2-(3-acetyl-4-hydroxy benzylidine)-6-hydroxy-4-methoxybenzofuran-3(2H)-one. And the structures of the isolate were elucidated via a combination of spectral techniques. Also study the antimicrobial activity of ethanol extract of R. rhabarbarum stems against bacterial and fungal strain. And the results showed that the ethanolic extract was very active against Staphylococcus aureus, Bacillus subtilis, and the fungus Candida albicans, and active against Pseudomonas aeruginosa, and inactive against Escherichia coli.

Keywords

Rheum rhabarbarum, medicinal herb, Flavonoid, Antimicrobial, TLC


Citation of this Article

Hala I Elzubir, Abdel Karim M, Asra Banu Syeda, Leena S Ibrahim, Fatima A Khleef, & Hiam M. Osman. (2026). Isolation of Flavonoid (Z)-2-(3-Acetyl-4-Hydroxy Benzylidine)-6-Hydroxy-4-Methoxybenzofuran-3(2h)-One of Rheum Rhabarbarum and Evaluation of Antimicrobial Activity against a Panel of Human Pathogens. International Research Journal of Innovations in Engineering and Technology - IRJIET, 10(4), 347-354. Article DOI https://doi.org/10.47001/IRJIET/2026.104049

Licence Copyright (c) 2026 International Research Journal of Innovations in Engineering and Technology creative common licence This work is licensed under Creative common Attribution Non Commercial 4.0 Internation Licence
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