Balanites Aegyptiaca is a medicinal plant used in Africa’s folk medicines to treat wide range of diseases. Phytochemical analysis of ethanolic extract of balanites aegyptiaca revealed the presence of flavonoids, Glycosides, Alkaloids, Tannins, Anthraquinones, Terpenoids, phenols, saponins. However, steroids was found to be absent. The plant was evaluated for potential antimicrobial activity against medically important bacterial and fungal strains. The antimicrobial activity was determined in the extracts using agar well diffusion method. The antibacterial and antifungal activities of extracts in different concentrations 5, 10, 15, and 20 mg/ml were tested against two Gram-positive Staphylococcus aureus, Staphylococcus Epidermis; two Gram-negative, Escherichia coli, Pseudomonas aeruginosa and two fungal strains, Aspergillus flavus, Candida albicans. The zones of inhibition of the extracts were compared with that of different standards like ampicillin 1mg/ml for antibacterial activity and fluconazole 1mg/ml for antifungal activity. The results showed that the extract exhibited a remarkable inhibition of the microbial growth against the tested organisms.