International Research Journal of Innovations in Engineering and Technology - IRJIET International Open Access, Monthly, Peer Reviewed, Reputed Journal ISSN (online): 2581-3048

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DOI Prefix: 10.47001/IRJIET

Evaluation of Antimicrobial Activity and Phytochemical Analysis of Ethanolic Leave Extract of Balanites Aegyptiaca

AbdulHamid UmarDepartment of Chemistry, Adamawa State University Mubi, PMB 25 Mubi, Adamawa State, Nigeria

Vol 7 No 7 (2023): Volume 7, Issue 7, July 2023 | Pages: 178-181

International Research Journal of Innovations in Engineering and Technology

OPEN ACCESS | Research Article | Published Date: 29-07-2023

doi Logo doi.org/10.47001/IRJIET/2023.707027

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Abstract

Balanites Aegyptiaca is a medicinal plant used in Africa’s folk medicines to treat wide range of diseases. Phytochemical analysis of ethanolic extract of balanites aegyptiaca revealed the presence of flavonoids, Glycosides, Alkaloids, Tannins, Anthraquinones, Terpenoids, phenols, saponins. However, steroids was found to be absent. The plant was evaluated for potential antimicrobial activity against medically important bacterial and fungal strains. The antimicrobial activity was determined in the extracts using agar well diffusion method. The antibacterial and antifungal activities of extracts in different concentrations 5, 10, 15, and 20 mg/ml were tested against two Gram-positive Staphylococcus aureus, Staphylococcus Epidermis; two Gram-negative, Escherichia coli, Pseudomonas aeruginosa and two fungal strains, Aspergillus flavus, Candida albicans. The zones of inhibition of the extracts were compared with that of different standards like ampicillin 1mg/ml for antibacterial activity and fluconazole 1mg/ml for antifungal activity. The results showed that the extract exhibited a remarkable inhibition of the microbial growth against the tested organisms.

Keywords

Phytochemical, Balanites Aegyptiaca, Antimicrobial Activity, Medicinal plant, Ethanolic extract


Citation of this Article

AbdulHamid Umar, “Evaluation of Antimicrobial Activity and Phytochemical Analysis of Ethanolic Leave Extract of Balanites Aegyptiaca” Published in International Research Journal of Innovations in Engineering and Technology - IRJIET, Volume 7, Issue 7, pp 178-181, July 2023. Article DOI https://doi.org/10.47001/IRJIET/2023.707027

Licence Copyright (c) 2026 International Research Journal of Innovations in Engineering and Technology creative common licence This work is licensed under Creative common Attribution Non Commercial 4.0 Internation Licence
References
  1. Wagini, N. H., Bello, A., Safana, A., Abubakar, S., Usman, H. B., Yusuf, A. M., and Muhammad, H. Phytochemical screening and antibacterial properties of henna (Lawsonia inermis) Roots extracts. Katsina Journal of Natural and Applied Sciences, 4(2), 151-160, 2015.
  2. Mallikharjuna, P. B., Rajanna, L. N., Seetharam, Y. N., and Sharanabasappa, G. K. Phytochemical studies of Strychnos potatorum Lf-A medicinal plant. Journal of chemistry, 4, 510-518, 2007.
  3. Soberon, J. R., Sgariglia, M. A., Sampietro, D. A., Quiroga, E. N., and Vattuone, M. A. Antibacterial activity of plant extracts from northwestern Argentina. Journal of Applied Microbiology, 102(6), 1450-1461, 2007.
  4. Usman, A., Mohammed, Y., Muhammed, H. O., and Zakari, A. H. (2010). Phytochemical screening and antioxidant activity of Balanites Aegyptiaca root bark extracts: influence of solvent. Communication in Physical Sciences, 5(1, 2, 3), 2007.
  5. Chothani, D. L., & Vaghasiya, H. U. A review on Balanites aegyptiaca Del (desert date): phytochemical constituents, traditional uses, and pharmacological activity. Pharmacognosy reviews, 5(9), 55, 2011.
  6. Hall J.B, Walker D.H.  Balanites aegyptiaca Del. a monograph. School of Agriculture and Forest Sciences. Banger: University of Wales, 980-910, 1991.
  7. Zarroug, I. M., Nugud, A. D., Bashir, A. K., and Mageed, A. A.  Balanites aegyptiaca as a mosquito larvicide. International Journal of Crude Drug Research, 28(4), 267-271, 1990.
  8. Vaghasiya, Y., R. Dave, and S. Chanda. "Phytochemical analysis of some medicinal plants from western region of India." Research Journal of Medicinal Plant 5, no. 5: 567-576, 2011.
  9. Sofowara A. Medicinal plants and traditional medicine in Africa (2nd edition). Screeningfor bioactive agents Spectrum books limited, Ibadan, Nigeria, 134-156 1993.
  10. Trease G.E, Evans W. C.Trease and Evans Mexican plants. Journal of ethano pharmacological pharmacognosy; 13: 222-230, 1989.
  11. Gummuluri, S., Kavalipurapu, V. T., and Kaligotla, A. V. Antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia against Enterococcus Feacalis by Agar Well Diffusion Method and Minimal Inhibitory Concentration-An Invitro Study. Brazilian Dental Science, 22(3), 365-370, 2019.
  12. Abdulhamid, A., and Sani, I. Preliminary phytochemical screening and antimicrobial activity of aqueous and methanolic leave extracts of Balanites aegyptiaca. International Research Journal of Pharmaceutical and Biosciences, 3(1), 1-7, 2016.
  13. Guil-Guerrero, J. L., Ramos, L., Moreno, C., Zúñiga-Paredes, J. C., Carlosama-Yepez, M., and Ruales, P. Antimicrobial activity of plant-food by-products: A review focusing on the tropics. Livestock Science, 189, 32-49, 2016.
  14. Akiyama, H., Fujii, K., Yamasaki, O., Oono, T., and Iwatsuki, K. Antibacterial action of several tannins against Staphylococcus aureus. Journal of antimicrobial chemotherapy, 48(4), 487-491, 2001.
  15. Haslam, E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action. Journal of natural products, 59(2), 205-215, 1996.

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