Generally, nanoparticles are prepared by a variety of chemical methods which are not environmentally friendly. Use of biological method (plant seeds) in the synthesis of nanoparticles is quite novel leading to truly green synthesis which provides advancement over chemical and physical method, as it is cost effective and environment friendly. The stable, green synthesis of silver nanoparticles through biological methods has a wide range of applications and is very gratifying in the medical field because of their low toxicity. Therefore, the demands of silver nanoparticles synthesized biologically are on the rise. The present work aims at Biosynthesis of Trigonella foenum-gracum L seed nanoparticles and their characterization. During the synthesis of silver nanoparticles, the reduction reaction takes place i.e., Ag+ to Ag0 during exposure to the sunlight. The confirmation of synthesized nanoparticles from Trigonella foenum-gracum L seed extract was confirmed visibly by color change of the solution from light yellow to deep brown within 30 min of exposure. The separation of these nanoparticles was performed by centrifugation. The pellet of silver nanoparticles was further converted to powder by drying. From the characterization studies conducted by UV-Visible spectroscopy, it is observed that surface Plasmon resonance peaks have shown the maximum absorbance of silver- nanoparticles at 425 nm, indicating that silver nanoparticles were synthesized. Further characterization was conducted by distinct types of methods mentioned such as scanning electron microscope (SEM) analysis, Fourier Transform Infrared Spectroscopy (FTIR) analysis and Elemental detection sensor (EDS) analysis. The average size was found to be 100 nm and sphere shaped evaluated by Scanning electron microscope (SEM). The Fourier Transform Infrared Spectroscopy (FTIR) spectrum revealed that the absorption bands at 3282 cm1 and 1639 cm1 indicated the presence of -OH or -COOH and amide group stretching in the silver nanoparticles. The Elemental detection sensor (EDS) images are characterized by spectrum process with number of iteration 2 and peak are omitted at 2.145, 5.740keV represents the presence of elements like Silver, Carbon and Oxygen.