ESTD Year: 2017 | Impact Factor: 6.9
DOI Prefix: 10.47001/IRJIET
Vol 10 No 8 (2026): Volume 10, Issue 8, August 2026 | Pages: 57-64
International Research Journal of Innovations in Engineering and Technology
OPEN ACCESS | Research Article | Published Date: 20-08-2026
This study assessed the anti-nutritional analysis and vitamins in Ribes nigrum (Blackcurrant) seed extracts using ethanol, N-hexane, and ethyl acetate as solvents. The primary objective was to identify the nutritional and therapeutic potential of the seeds through standard analytical and spectroscopic techniques. The samples were extracted by solvent extraction, after which quantitative phytochemical analysis was carried out following AOAC (2016) standard methods. Vitamin and mineral contents were analysed using UV–Visible spectrophotometry and Atomic Absorption Spectrophotometry (AAS), respectively. Fourier Transform Infrared Spectroscopy (FTIR) was employed to identify the functional groups present in the extracts, while Gas Chromatography–Mass Spectrometry (GC–MS) was used to identify individual bioactive components. The results showed that Ribes nigrum seeds are rich in flavonoids, phenols, tannins, alkaloids, steroids, terpenoids, and glycosides, which contribute to their strong antioxidant and medicinal properties. The mineral analysis indicated high levels of potassium (385.06 mg/100 g), calcium (281.06 mg/100 g), and magnesium (197.42 mg/100 g), all essential for physiological functions. Vitamin analysis showed significant concentrations of vitamins A, B-complex, C, D, E, and K, confirming the nutritional value of the seed. The FTIR spectra confirmed the presence of key functional groups such as O–H, C–H, C=O, C–O, and C=C, indicating alcohols, esters, phenolics, and aromatic compounds. GC–MS analysis revealed several major bioactive compounds including oleic acid, linoleic acid, n-hexadecanoic acid, squalene, methyl stearate, and cis-vaccenic acid, known for their antioxidant, anti-inflammatory, and antimicrobial activities. The study demonstrates that Ribes nigrum seeds contain a wide range of nutritional and bioactive compounds with potential applications in food, nutraceutical, pharmaceutical, and cosmetic industries. Further research is recommended to isolate and characterize specific compounds responsible for the observed biological activities and to evaluate their toxicological safety for therapeutic use.
Anti-nutritional, Vitamins, Ribes Nigrum, Blackcurrant Seeds, Anti-Nutritional Factors, Phytochemical Analysis, Vitamins, Mineral Composition, FTIR, GC–MS, Bioactive Compounds, Antioxidant Activity, Nutraceutical Potential, Medicinal Plants.
Anami Paul Abu, Williams E.T., & Priscilla Alexander. (2026). Anti-Nutritional and Vitamins Analysis of Ribes Nigrum Seed Extracts in Mubi North Local Government Area Adamawa State, Nigeria. International Research Journal of Innovations in Engineering and Technology - IRJIET, 10(8), 57-64. Article DOI https://doi.org/10.47001/IRJIET/2026.108007
This work is licensed under Creative common Attribution Non Commercial 4.0 Internation Licence
Ajayi, O. A., and Osho, I. B. (2021). Mineral and vitamin assessment of Ficusreligiosa seeds from Nigeria. Nigerian Journal of Applied Science, 39(2), 89–98.
Akhtar, N., Mehmood, A., and Khan, M. S. (2021). Phytochemical and spectral analysis of Ribes nigrum seed extracts. Journal of Medicinal Plants Research, 15(6), 256–265.
Amanullah, M., Khalid, A., Rahman, I. U., Ali, N., Ahmad, H., and Khan, A. (2022). Proximate composition and nutritional value of selected edible plant seeds from tropical regions. Journal of Food Studies, 11(2), 45–56. https://doi.org/10.5296/jfs.v11i2.20145
Aremu, M. O., Olaofe, O., and Akintayo, E. T. (2021). Nutritional composition of selected.
Ibrahim, M. A., Abdullahi, I. O., and Aliyu, A. M. (2019). UV–Visible and FTIR characterization of Citrulluscolocynthis seed extracts. African Journal of Biotechnology, 18(12), 289–297.
Ibrahim, U. A., Musa, M. B., and Lawal, M. (2019). Nutritional evaluation of Moringaoleifera seed flour and oil. Journal of Food and Chemical Sciences, 11(2), 102–110.
Jamilu, M. A., Ibrahim, I. Y., and Musa, S. M. (2022). Phytochemical screening and biological activities of ethanolic extracts of medicinal plants used in Hausa traditional medicine. West African Journal of Biological Sciences, 8(1), 44–52.
Jenkins, R. (1999). X-Ray Fluorescence Spectrometry. Wiley.
Jodeh, S., Jaradat, N., Hammad, J., Hamed, O., and Abualhasan, M. (2021). Phytochemical screening and antioxidant activity of various plant seeds. Journal of Food Measurement and Characterization, 15(4), 3112–3123.
Kaume, L., Howard, L. R., and Devareddy, L. (2012). The Blackberry Fruit: A Review on Its Composition and Chemistry, Metabolism and Bioavailability, and Health Benefits. Journal of Agricultural and Food Chemistry, 60(23), 5716-5727.
Kaume, L., Howard, L. R., and Devareddy, L. (2012). The blackberry fruit: Review of its composition, antioxidant properties, and health benefits. Journal of Agricultural and Food Chemistry, 60(23), 5716–5727. https://doi.org/10.1021/jf204665b
Konopka, I., Tańska, M., and Zadernowski, R. (2023). Characteristics of blackcurrant seeds as valuable sources of nutrients and bioactives. Applied Sciences, 13(23), 12829.
Kumar, R., Singh, A., and Sharma, V. (2023). Spectroscopic evaluation of bioactive plant extracts and their antioxidant properties. International Journal of Analytical Chemistry, 2023, 1–12.
Kumar, S., Gupta, D., and Singh, A. (2023). Spectral fingerprinting and antioxidant properties of Ribesnigrumseed extract. Plant Biochemistry and Phytotechnology, 18(3), 121–133.
Mazza, G., and Miniati, E. (2018). Anthocyanins in Fruits, Vegetables, and Grains. CRC Press.
Mikkonen, T. P., Kokko, H. I., and Oksman-Caldentey, K. M. (2020). Nutritional and functional aspects of blackcurrant polyphenols: A review. Food Chemistry, 302, 125345. https://doi.org/10.1016/j.foodchem.2019.125345.
Mikkonen, T. P., Yli-Jokipii, K. M., and Kallio, H. P. (2022). Development of blackcurrant-based nutraceuticals: Composition, antioxidant potential, and physiological effects. Food Research International, 156, 111166.
Miner, A., Kanaan, A., Ibrahim, M., and Soliman, F. (2024). Environmental variations and their influence on mineral composition of plant seeds: A multidisciplinary assessment. Open Agriculture, 18(1), 1–9. https://doi.org/10.1515/opag-2024-0001
Nwofia, G. E., Edeoga, H. O., and Nwogu, L. A. (2020). Macro- and micro-mineral content of selected tropical plant seeds. Plant Foods for Human Nutrition, 75(4), 589–597.
Pokharel, U., Acharya, P., and Bhattarai, S. (2024). Anti-nutritional factors and their effects on nutrient bioavailability in plant foods. Foods, 13(4), 1–12.
Pokharel, U., Shah, S., and Adhikari, P. (2024). Anti-nutritional factors in edible plants and their effects on nutrient bioavailability: An updated review. Foods, 13(4), 112–130. https://doi.org/10.3390/foods13040112
Usman, M. K. (2021). Biological and therapeutic significance of alkaloids in medicinal plants. Journal of Pharmacognosy Research, 13(2), 120–132.
Okolie, P. N., Eze, J. I., and Nwachukwu, C. U. (2022). Vitamins and antioxidant properties of selected fruit seeds. Food Research International, 157, 111456.
Olayemi, F. O., Ojo, D. A., and Adeola, A. A. (2020). Comparative UV–Vis and FTIR analysis of Moringaoleifera leaves and seed extracts. Scientific African, 8, e00416.
Solcan, M., Cioanca, O., Hăncianu, M., and Miron, A. (2023). Biochemical, nutritional, and antioxidant profiling of Ribes nigrum fruits and seeds. Plants, 12(11), 2456. https://doi.org/10.3390/plants12112456.
Stein, S. E. (1999). An integrated method for spectrum extraction and compound identification from gas chromatography/mass spectrometry data. Journal of the American Society for Mass Spectrometry, 10(8), 770-781.
Suk, J. H., Park, Y., and Kim, S. (2021). Therapeutic significance of palmitoleic acid: A review. Nutrients, 13(3), 1044.
Suleiman, H. A. (2023). UV-visible spectroscopic identification of flavonoids and phenols in plant extracts. Spectroscopy Today, 12(2), 201–213.
Swartz, H. J. (2021). The cultural symbolism and folk uses of Ribes nigrum in Northern Europe. Ethnobotany Research and Applications, 22(3), 89–101.
Tang, X., Zhao, J., and Yang, J. (2022). Nutritional and biochemical relevance of oleic acid in human health. Frontiers in Nutrition, 9, 874202.
Teleszko, M., and Wojdyło, A. (2015). Bioactive compounds and antioxidant activity of blackcurrant and black chokeberry. Food Chemistry, 173, 471–480. https://doi.org/10.1016/j.foodchem.2014.09.070
Tian, H., Zhang, Y., and Wei, X. (2021). Characterization of lipid-derived volatiles in seed oils by GC–MS and their biological relevance. Food Research International, 149, 110691.
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., and Kaur, H. (2011). Phytochemical screening and extraction: A review. International Pharmaceutica Sciencia, 1(1), 98–106.
Torkova, A. A., Kolesnikova, O. I., and Khramova, D. S. (2020). Antioxidant activity and polyphenol content in blackcurrant berries. Food Chemistry, 325, 126859.
Traber, M. G., and Stevens, J. F. (2011). Vitamins C and E: beneficial effects from a mechanistic perspective. Free Radical Biology and Medicine, 51(5), 1000-1013.
Traber, M. G., and Stevens, J. F. (2011). Vitamins C and E: Beneficial effects from a mechanistic perspective. Free Radical Biology and Medicine, 51(5), 1000–1013. https://doi.org/10.1016/j.freeradbiomed.2011.05.017
Trease, G. E., and Evans, W. C. (2002). Pharmacognosy (15th ed.). Saunders Publishers, London.
Usman, L. A., Danladi, K. I., and Lawal, A. O. (2022). Spectral and phytochemical evaluation of Carica papaya seeds and its pharmacological potential. Journal of Applied Sciences and Environmental Management, 26(2), 261–269.
WilliamsE. T., Timothy N. and Chika A.(2019). Phytochemical Screening, Elemental and Proximate Analysis of Maeruaangolensis (Capparaceaea). Stem Bark International Journal of Biochemistry Research & Review, 27 (4): 1-10.
Williams, E.T. and Lenkat I. D. (2018). Proximate Composition and some Elemental Analysis of Watermelon Seed (Citrulluslanatus thumb). Adamawa State University Journal of Scientific Research ISSN: 2251-0702 (P) Volume 6 Number 1, April, 2018, Article no. ADSUJSR 0601019 http://www.adsujsr.com
WHO/FAO. (2011). Joint FAO/WHO Expert Consultation on Human Vitamin and Mineral Requirements. Geneva: World Health Organization.
World Health Organization (WHO). (2011). Guidelines for the Assessment of Food Quality and Safety. Geneva: WHO Press.
Wójciak, M. (2022). Phytochemical constituents and functional benefits of edible plant seeds: A comprehensive review. Foods, 11(9), 1378. https://doi.org/10.3390/foods11091378
Varela, M. R., Fernández, R. I., and Soto, S. (2023). Health-promoting properties and traditional applications of blackcurrant (Ribes nigrum L.): An updated overview. Journal of Ethnopharmacology, 312, 116428.